Journal: Journal of Cellular and Molecular Medicine

Article Title: Interleukin-33 overexpression is associated with liver fibrosis in mice and humans

doi: 10.1111/j.1582-4934.2009.00801.x

Figure Lengend Snippet: Antibodies used

Article Snippet: FITC-conjugated mouse monoclonal IgG1 anti-human ST2L (clone B4E6) , 10 μq/ml , MD Biosciences (Zürich, Switzerland).

Techniques:

Journal: BMC Immunology

Article Title: Interleukin-33 contributes to both M1 and M2 chemokine marker expression in human macrophages

doi: 10.1186/1471-2172-11-52

Figure Lengend Snippet: ST2, membrane bound (ST2L), and soluble (s) ST2 transcript levels in M1 and M2 polarized human bone marrow-derived macrophages . ( A ) Transcript levels of ST2, ST2L, and sST2 from macrophages exposed to M1 polarizing mediators. ( B ) Transcript levels of ST2, ST2L, and sST2 from macrophages exposed to M2 polarizing mediators. Results are expressed as fold increase in transcript levels compared with levels of these transcripts in human macrophages exposed to media alone (i.e. control condition). Each symbol denotes a single donor (n = 5-14 donors); mean and SEM are also shown.

Article Snippet: Employing a standard immunocytochemistry technique, an anti-ST2 polyclonal antibody from ProSci (Poway, CA) was used to stain for ST2 in primary human macrophages exposed to media alone, IL-33, M1-polarizing mediators, or M2-polarizing mediators.

Techniques: Membrane, Derivative Assay, Control

Journal: BMC Immunology

Article Title: Interleukin-33 contributes to both M1 and M2 chemokine marker expression in human macrophages

doi: 10.1186/1471-2172-11-52

Figure Lengend Snippet: ST2 protein expression in human bone marrow-derived macrophages . ( A-H ) Immunostaining of human macrophages cultured on Lab-Tek slides after exposure to medium alone (A & E), IL-33 (B & F), M1 polarizing mediators (C & G), or M2 polarizing mediators (D & H). IgG controls are shown in panels A, B, C, & D. ( I ) Western blotting for ST2L and sST2 protein was performed in protein extracts from naïve and M2-polarized human macrophages; CE = cytoplasmic extract and NE = nuclear extract. ( J ) Quantitative analysis of ST2L and sST2 protein band intensity.

Article Snippet: Employing a standard immunocytochemistry technique, an anti-ST2 polyclonal antibody from ProSci (Poway, CA) was used to stain for ST2 in primary human macrophages exposed to media alone, IL-33, M1-polarizing mediators, or M2-polarizing mediators.

Techniques: Expressing, Derivative Assay, Immunostaining, Cell Culture, Western Blot